APC Anti-Human CD61 (VIPL2)

CD61 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

VIPL2

Cat no : APC-65185

Synonyms

CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3

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Validation Data Gallery

• 

  FC experiment of human peripheral blood platelets using APC-65185

  1X10^6 human peripheral blood platelets were surface stained with 10.00 ul/test APC Anti-Human CD61 (APC-65185, Clone:VIPL2) (red) or isotype control antibody (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood platelets

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65185 targets CD61 in FC applications and shows reactivity with Human, Non-human primates samples.

• Tested Reactivity: Human, Non-human primates
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
• Calculated Molecular Weight: 788 aa, 87 kDa
• GenBank Accession Number: BC127666
• Gene Symbol: ITGB3
• Gene ID (NCBI): 3690
• RRID: AB_2883045
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Storage Buffer: PBS with 0.1% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD61 is a 110-kDa membrane glycoprotein that is also termed integrin β3 chain or GPIIIa. CD61 can associate with integrin αV chain (CD51) or integrin αIIb chain (CD41, GPIIb) to form heterodimeric receptors for cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, plasminogen, prothrombin, thrombospondin, vitronectin and von Willebrand factor (PMID: 7694604; 9251239; 2018971; 20628538). Integrin αVβ3 (CD51-CD61) is present on endothelium, smooth muscle, osteoclasts, platelets, some fibroblasts, some activated leukocytes and tumor cells (PMID: 7694604). GPllb-llla (CD41-CD61) is restricted in distribution to platelets and cells of megakaryoblastic potential and is required for platelet aggregation (PMID: 2018971; 16322781).