Anti-Mouse CD62L (L-Selectin) (MEL-14)

CD62L (L-Selectin) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

2

CloneNo.

MEL-14

Cat no : 65123-1-Ig

Synonyms

CD62L (L-Selectin), Sell

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 65123-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 0.5 ug Anti-Mouse CD62L (L-Selectin) (65123-1-Ig, Clone: MEL-14) and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65123-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface stained with 5 ul CoraLite® Plus 647-conjugated Anti-Mouse CD3 (CL647-65077, Clone: 17A2), and 0.5 ug Rat IgG2a Isotype Control and FITC Anti-Rat IgG2a Antibody at dilution 1:200. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

65123-1-Ig targets CD62L (L-Selectin) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: C3H/eb mouse B lymphoma 38C-13
• Full Name: selectin, lymphocyte
• GenBank Accession Number: BC052681
• Gene Symbol: CD62L
• Gene ID (NCBI): 20343
• RRID: AB_2918417
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: Phosphate based buffer with 0.09% sodium azide, pH 7.2.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).

Publications

Species	Application	Title
mouse	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
mouse	FC	Nat Nanotechnol; A bispecific nanosystem activates endogenous natural killer cells in the bone marrow for haematologic malignancies therapy; Authors - Yanqin Zhang