CD64 Polyclonal antibody

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  WB analysis using 32060-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32060-1-AP (CD64 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

CD64 Polyclonal Antibody for WB, ELISA

Cat No. 32060-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

IgG Fc receptor I, High affinity immunoglobulin gamma Fc receptor I, FcRI, Fcgr1, Fc-gamma RI

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Tested Applications

• Positive WB detected in: J774A.1 cells, RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32060-1-AP targets CD64 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: Fc receptor, IgG, high affinity I
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_010186.5
• Gene Symbol: Fcgr1
• Gene ID (NCBI): 14129
• RRID: AB_3670181
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P26151
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.