Anti-Mouse CD68 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of mouse peritoneal macrophages using 98029-1-RR (same clone as 98029-1-PBS)

  1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR, Clone: 230504G5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Mouse CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98029-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse peritoneal macrophages using 98029-1-RR (same clone as 98029-1-PBS)

  1x10^6 mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse CD68 Rabbit Recombinant Antibody (98029-1-RR, Clone: 230504G5) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 98029-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98029-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98029-1-RR against Mouse CD68 were performed. The affinity constant is 10.8 nM.

CD68 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98029-1-PBS

Clone No.230504G5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

230504G5, CD68 antigen, gp110, Scard1

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Product Information

98029-1-PBS targets CD68 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0787

  Product name: Recombinant mouse CD68 protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 21-291 aa of NM_001291058.1

  Sequence: DCPHKKAVTLLPSFTMTPTATESTASPTTSHRPTTTSHGNVTVHTSSGPTTVTHNPATTTSHGNATISHATVSPTTNGTATSPRSSTVGPHPGPPPPSPSPRSKGALGNYTWANGSQPCVQLQAQIQIRILYPIQGGRKAWGISVLNPNKTKVQGGCDGTHPHLSLSFPYGQLTFGFKQDLHQSPSTVYLDYMAVEYNVSFPQAAQWTFMAQNSSLRELQAPLGQSFCCGNASIVLSPAVHLDLLSLRLQAAQLPDKGHFGPCFSCNRDQS

• Full Name: CD68 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_001291058.1
• Gene Symbol: Cd68
• Gene ID (NCBI): 12514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P31996-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mouse CD68 (also known as macrosialin) is a type I transmembrane glycoprotein that is highly and specifically expressed by mouse tissue macrophages, and to a lesser extent by dendritic cells. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.