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CD69 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 10803-1-AP

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Con: 36 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

CD69 Antibody WB BxPC-3 cells 10803-1-AP

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10803-1-AP(CD69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours

CD69 Antibody IHC human tonsillitis tissue 10803-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP( CD69 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CD69 Antibody IHC human tonsillitis tissue 10803-1-AP

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10803-1-AP( CD69 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human
Positive WB detected in:
BxPC-3 cells
Positive IHC detected in:
human tonsillitis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CD69 fusion protein Ag1094
Full name:
CD69 molecule
Calculated molecular weight:
20 kDa
Observed molecular weight:
60 kDa
GenBank accession number:
BC007037
Gene ID (NCBI):
969
Gene symbol
CD69
Synonyms
Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3

Background

CD69, also known as activation inducer molecule (AIM) or early activation antigen (EA1), is a cell surface glycoprotein expressed shortly after activation on T lymphocytes and other cells of haematopoietic origin, including natural killer cells. It is a disulfide-linked homodimer composed of two chains belonging to the type II integral protein with a C-type lectin domain. CD69 is involved in lymphocyte proliferation and functions as a signal-transmitting receptor in lymphocytes, natural killer cells, and platelets.


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