Tested Applications
| Positive FC detected in | Anti-CD3/CD28-stimulated mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65105-1-Ig targets CD69 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Armenian Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Mouse dendritic epidermal T cell line Y245 Predict reactive species |
| Full Name | CD69 antigen |
| GenBank Accession Number | BC106997 |
| Gene Symbol | Cd69 |
| Gene ID (NCBI) | 12515 |
| RRID | AB_2918404 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P37217 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CD69 antibody 65105-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse CD69 (H1.2F3), catalog number 65105-1-Ig, has 2 published citations. They appear in Advanced Materials (IF 29.1) and International Journal of Biological Macromolecules (IF 8.7). Both studies used flow cytometry in mouse models. The research fields span oncology (sonodynamic therapy for solid tumor eradication) and immunology (intestinal mucosal and systemic immunity enhancement).
| Species | Application | Title |
|---|---|---|
Adv Mater Ultrasound-Activated Sonophage Synergizes Sonodynamic Therapy and Saltoptosis for Solid Tumor Eradication. | ||
Int J Biol Macromol The impact of novel oral adjuvant dendritic fibrous nano-silica coated with M cell homing peptide-modified chitosan on enhancing both the intestinal mucosal and systemic immunity. |
