Anti-Human CD69 Rabbit Recombinant Antibody

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• Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR)

CD69 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98173-1-RR Copied!

Clone No. 241552F1

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

• 

  FC experiment of human PBMCs using 98173-1-RR

  1x10^6 untreated or PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD4 (RPA-T4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using 98173-1-RR

  1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1) (red) or 0.25 ug Isotype Control, or 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD69 Rabbit Recombinant Antibody (98173-1-RR, Clone:241552F1)(black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 98173-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98173-1-RR against Human CD69 were performed. The affinity constant is 0.257 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: PHA treated human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98173-1-RR targets CD69 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  FusionProtein

• Full Name: CD69 molecule
• Calculated Molecular Weight: 23 kDa
• GenBank Accession Number: NM_001781.2
• Gene Symbol: CD69
• Gene ID (NCBI): 969
• RRID: AB_3672316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q07108
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

Publications

What published studies show

The Anti-Human CD69 Rabbit Recombinant Antibody (clone 241552F1), catalog number 98173-1-RR, has 2 total citations. The retrieved citation appears in the Journal of Pharmaceutical Analysis (J Pharm Anal, impact factor 11.2), published in 2026. The associated research field is immunology and antitumor immunity, specifically a proteolysis-targeting chimera (PROTAC) strategy targeting multiple immune checkpoints containing ITIMs. The antibody was applied in flow cytometry (FC) using human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	FC	J Pharm Anal; A novel proteolysis-targeting chimera strategy targeting multiple immune checkpoints containing ITIMs enhances antitumor immunity.; Authors - Yue-Yuan Qiu