Tested Applications
| Positive FC detected in | Anti-CD3/CD28 treated mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 3 publications below |
Product Information
FITC-65105 targets CD69 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Mouse dendritic epidermal T cell line Y245 Predict reactive species |
| Full Name | CD69 antigen |
| GenBank Accession Number | BC106997 |
| Gene Symbol | Cd69 |
| Gene ID (NCBI) | 12515 |
| RRID | AB_2883777 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P37217 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.1% gelatin, pH 7.2. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD69 antibody FITC-65105 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Nanotechnol A bispecific nanosystem activates endogenous natural killer cells in the bone marrow for haematologic malignancies therapy | ||
Adv Healthc Mater Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization | ||
Int J Biol Macromol Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization |
