CD71 Polyclonal antibody, PBS Only

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  WB analysis using 30574-1-AP (same clone as 30574-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30574-1-AP (CD71 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 30574-1-AP (same clone as 30574-1-PBS)

  IP result of anti-CD71 (IP:30574-1-AP, 4ug; Detection:30574-1-AP 1:10000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 30574-1-AP (same clone as 30574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30574-1-AP (CD71 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 30574-1-AP (same clone as 30574-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30574-1-AP (CD71 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 30574-1-AP (same clone as 30574-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD71 antibody (30574-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 30574-1-AP (same clone as 30574-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (30574-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30574-1-PBS in a different storage buffer formulation.

CD71 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 30574-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

p90, sTfR, T9, TFR, TFR1

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Product Information

30574-1-PBS targets CD71 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD71 fusion protein Eg0339
• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 80-90 kDa
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• ENSEMBL Gene ID: ENSG00000072274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P02786
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.