CD71 Recombinant antibody

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  WB analysis using 84766-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84766-4-RR (Transferrin R antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 84766-4-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD71 antibody (84766-4-RR, Clone: 242122B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84766-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84766-4-RR against Mouse CD71 were performed. The affinity constant is 1.04 nM.

CD71 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84766-4-RR

Clone No.242122B2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Tfrc, transferrin receptor, TR, TFR1, TFR

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, mouse placenta tissue
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84766-4-RR targets CD71 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: transferrin receptor
• Calculated Molecular Weight: 86kDa
• Observed Molecular Weight: 85-100 kDa
• GenBank Accession Number: NM_011638.4
• Gene Symbol: CD71
• Gene ID (NCBI): 22042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q62351
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.