Tested Applications
| Positive WB detected in | RAW 264.7 cells, mouse placenta tissue |
| Positive IF/ICC detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84766-4-RR targets CD71 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | transferrin receptor |
| Calculated Molecular Weight | 86kDa |
| Observed Molecular Weight | 85-100 kDa |
| GenBank Accession Number | NM_011638.4 |
| Gene Symbol | CD71 |
| Gene ID (NCBI) | 22042 |
| RRID | AB_3743712 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q62351 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD71 antibody 84766-4-RR | Download protocol |
| WB protocol for CD71 antibody 84766-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD71 Recombinant monoclonal antibody (242122B2), catalog number 84766-4-RR, has 12 citations. These appear in journals including Gut Microbes, Phytomedicine, Mater Today Bio, Biomed Pharmacother, Front Immunol, Toxicology, Ann Med, Cell Signal, J Cereb Blood Flow Metab, and Exp Cell Res. The research fields center on ferroptosis, spanning liver disease, sarcopenia, osteoarthritis, diabetes, non-small cell lung cancer, colorectal cancer, pulmonary hypertension, sepsis, and intracranial aneurysm.
| Species | Application | Title |
|---|---|---|
Gut Microbes Lachnospiraceae bacterium alleviates alcohol-associated liver disease by enhancing N-acetyl-glutamic acid levels and inhibiting ferroptosis through the KEAP1-NRF2 pathway | ||
Phytomedicine Cistanche deserticola extract and its active components, echinacoside, ameliorate sarcopenia by activating the IGF-1/PI3K-AKT pathway to modulate ferroptosis. | ||
Phytomedicine Multi-omics reveals that 4'-O-β-D-glucosyl-5-O-methylvisamminol ameliorates acute liver injury by modulating the miR-30e-3p/Tfrc axis and a multi-dimensional network of ferroptosis, inflammation, metabolism, and gut microbiota. | ||
Mater Today Bio Application of a novel myristoylproteomics approach identifies GLIPR2 as a key pro-ferroptotic substrate in non-small cell lung cancer. | ||
Biomed Pharmacother p16INK4a downregulation alleviates temporomandibular joint osteoarthritis combined with type 2 diabetes by driving M2 polarization | ||
Front Immunol Vitamin D ameliorates prediabetic cardiac injure via modulation of the ErbB4/ferroptosis signaling axis. |
