Mouse CD72 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of mouse spleen using 85208-5-RR (same clone as 85208-5-PBS)

  mouse spleen tissue was subjected to SDS PAGE followed by western blot with 85208-5-RR (Cd72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85208-5-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 85208-5-RR (same clone as 85208-5-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85208-5-RR (Cd72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85208-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 85208-5-RR (same clone as 85208-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 85208-5-RR (CD72 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85208-5-PBS in a different storage buffer formulation.

CD72 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85208-5-PBS

Clone No.242846B5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

242846B5, B-cell differentiation antigen CD72, Ly32, Ly-32, Lyb-2

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Product Information

85208-5-PBS targets CD72 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2708

  Product name: Recombinant Mouse Cd72 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 117-354 aa of NM_007654.2

  Sequence: RYLQVSRQFQEGTRIWEATNSSLQQQLREKISQLGQKEVELQKARKELISSQDTLQEKQRTHEDAEQQLQACQAERAKTKENLKTEEERRRDLDQRLTSTRETLRRFFSDSSDTCCPCGWIPYQERCFYISHTLGSLEESQKYCTSLSSKLAAFDEPSKYYYEVSLPSGLEELLDRSKSYWIQMSKKWRQDSDSQSRHCVRIKTYYQKWERTISKCAELHPCICESEAFRFPDGINLN

• Full Name: CD72 antigen
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: NM_007654.2
• Gene Symbol: Cd72
• Gene ID (NCBI): 12517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21855
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).