Anti-Mouse CD72 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of mouse splenocytes using 98354-3-RR (same clone as 98354-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD72 Rabbit RecAb (98354-3-RR, Clone: 242846D10) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 750 Anti-Mouse CD19. Cells were not fixed. This data was developed using the same antibody clone with 98354-3-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98354-3-RR (same clone as 98354-3-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD72 Rabbit RecAb (98354-3-RR, Clone: 242846D10) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98354-3-PBS in a different storage buffer formulation.

CD72 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98354-3-PBS

Clone No.242846D10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

B-cell differentiation antigen CD72, Ly32, Ly-32, Lyb-2, Lymphocyte antigen 32

---

Product Information

98354-3-PBS targets CD72 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: CD72 antigen
• Calculated Molecular Weight: 40 kDa
• GenBank Accession Number: NM_007654.2
• Gene Symbol: Cd72
• Gene ID (NCBI): 12517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21855
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236).