CD73 Polyclonal antibody

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  WB analysis using 32131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32131-1-AP (CD73 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 32131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32131-1-AP (CD73 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 32131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32131-1-AP (CD73 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD73 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32131-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, ELISA

Synonyms

5'-deoxynucleotidase, 5'-NT, 5'-nucleotidase, EC:3.1.3.99, Ecto-5'-nucleotidase

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32131-1-AP targets CD73 in WB, IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD73 fusion protein Eg1389
• Full Name: 5' nucleotidase, ecto
• Calculated Molecular Weight: 64kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: NM_011851.4
• Gene Symbol: Nt5e
• Gene ID (NCBI): 23959
• RRID: AB_3670201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q61503
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).