CD73 Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 86512-3-RR (same clone as 86512-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86512-3-RR (CD73 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86512-3-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02481-2

  The mean CD73 concentration was determined to be 39.19 ng/mL in U-87 MG cell extract based on a 1.50 mg/mL extract load and 31.20 ng/mL in HT-29 cell extract based on a 1.10 mg/mL extract load.

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  Sandwich ELISA sample test of MP02481-2

  Plasma of six individual healthy human donors was measured. The CD73 concentration of detected samples was determined to be 17.66 ng/mL with a range of 9.53-35.23 ng/mL.

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  Sandwich ELISA standard curve of MP02481-2

  Sandwich ELISA standard curve of MP02481-2, Human CD73 Recombinant Matched Antibody Pair - PBS only. 86512-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2974. 86512-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  IF Staining of U-251 using 86512-3-RR (same clone as 86512-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CD73 antibody (86512-3-RR, Clone: 251250H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86512-3-PBS in a different storage buffer formulation.

CD73 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Cat No. 86512-3-PBS

Clone No.251250H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Synonyms

NT5E, 5 NT, 5 nucleotidase, 5' nucleotidase, ecto (CD73), 5'-deoxynucleotidase

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Product Information

86512-3-PBS targets CD73 as part of a matched antibody pair:

MP02481-2: 86512-5-PBS capture and 86512-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2974

  Product name: Recombinant Human CD73 protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 27-547 aa of NM_002526.3

  Sequence: WELTILHTNDVHSRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQGTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFPILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPGTNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDVVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLGYLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQELGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHTDEMFWNHVSMCILNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLKMDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVIYPAVEGRIK

• Full Name: 5'-nucleotidase, ecto (CD73)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: NM_002526.3
• Gene Symbol: CD73
• Gene ID (NCBI): 4907
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21589-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa, 64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).