Anti-Mouse CD73 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of mouse splenocytes using 98199-1-RR (same clone as 98199-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR, Clone: 241541G9) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98199-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98199-1-RR (same clone as 98199-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR, Clone: 241541G9) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98199-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98199-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98199-1-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

CD73 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98199-1-PBS

Clone No.241541G9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

241541G9, 5'-deoxynucleotidase, 5'-NT, 5'-nucleotidase, EC:3.1.3.99

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Product Information

98199-1-PBS targets CD73 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1389

  Product name: Recombinant Mouse 5'-Nucleotidase/CD73 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 29-551 aa of NM_011851.4

  Sequence: WELTILHTNDVHSRLEQTSDDSTKCLNASLCVGGVARLFTKVQQIRKEEPNVLFLDAGDQYQGTIWFTVYKGLEVAHFMNILGYDAMALGNHEFDNGVEGLIDPLLRNVKFPILSANIKARGPLAHQISGLFLPSKVLSVGGEVVGIVGYTSKETPFLSNPGTNLVFEDEISALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDIVVGGHSNTFLYTGNPPSKEVPAGKYPFIVTADDGRQVPVVQAYAFGKYLGYLKVEFDDKGNVITSYGNPILLNSSIPEDATIKADINQWRIKLDNYSTQELGRTIVYLDGSTQTCRFRECNMGNLICDAMINNNLRHPDEMFWNHVSMCIVNGGGIRSPIDEKNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHSVHRYGQSTGEFLQVGGIHVVYDINRKPWNRVVQLEVLCTKCRVPIYEPLEMDKVYKVTLPSYLANGGDGFQMIKDELLKHDSGDQDISVVSEYISKMKVVYPAVEGRIKFS

• Full Name: 5' nucleotidase, ecto
• Calculated Molecular Weight: 64kDa
• GenBank Accession Number: NM_011851.4
• Gene Symbol: Nt5e
• Gene ID (NCBI): 23959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q61503
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD73 is a membrane-bound enzyme that catalyzes the extracellular hydrolysis of adenosine monophosphate (AMP) to produce adenosine. It is expressed on various immune cells, including T cells, B cells, dendritic cells, and regulatory T cells (Tregs). CD73 works in conjunction with CD39 to break down extracellular ATP into adenosine, creating an immunosuppressive environment that can inhibit the function of cytotoxic T cells and natural killer (NK) cells. This process is essential for immune tolerance and the resolution of inflammation. CD73 is also an important player in controlling tumor progression.