CD74 Recombinant antibody

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  WB analysis using 85802-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85802-1-RR (CD74 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CD74 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85802-1-RR

Clone No.250109F2

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Class-II-associated invariant chain peptide, CLIP, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii

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Tested Applications

• Positive WB detected in: A20 cells, RAW 264.7 cells, mouse small intestine tissue, mouse lung tissue, mouse thymus tissue, mouse spleen tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85802-1-RR targets CD74 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD74 fusion protein Eg2716
• Full Name: CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: NM_001042605.1
• Gene Symbol: Cd74
• Gene ID (NCBI): 16149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04441-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD74, also known as MHC class II invariant chain Ii or HLA-DR-associated invariant chain, is a non-polymorphic type II transmembrane glycoprotein. CD74 is mainly distributed in B cells, monocytes, Langerhans cells, dendritic cells and thymic epithelial cells, and is also expressed in epithelial cells. CD74 plays a key role in MHC class II antigen processing and also serves as a cell surface receptor for the macrophage cytokine MIF. CD74 plays an important role in cardiovascular diseases such as atherosclerosis, ischemic heart disease, and myocardial hypertrophy.(PMID: 15475450; PMID: 27752708; PMID: 38992165; PMID: 36712241).