CD74 Recombinant antibody, PBS Only (Capture/Detector)

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  Sandwich ELISA standard curve of MP02221-2

  Sandwich ELISA standard curve of MP02221-2, Human CD74 Recombinant Matched Antibody Pair - PBS only. 86023-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1945. 86023-4-PBS was HRP conjugated as the detection antibody. Range: 125-8000 pg/mL.

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  Sample test of MP02221-1

  The mean CD74 concentration was determined to be 28.6 ng/mL in human serum.

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  Sample test of MP02221-2

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD74 concentration was undetectable in unstimulated PBMC supernatant, 3,681.7 pg/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP02221-2

  The mean CD74 concentration was determined to be 2,833.0 ng/mL in Raji cell extract based on a 1.8 mg/mL extract load.

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  Sample test of MP02221-2

  Serum of nine individual healthy human donors was measured. The CD74 concentration of detected samples was determined to be 732.3 pg/mL with a range of 232.3-2,400.9 pg/mL.

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  Cytometric bead array standard curve of MP02221-1

  Cytometric bead array standard curve of MP02221-1, CD74 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86023-2-PBS. Detection antibody: 86023-1-PBS. Standard: Eg1945. Range: 1.563-200 ng/mL.

CD74 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 86023-1-PBS

Clone No.250520A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Class-II-associated invariant chain peptide, DHLAG, HLADG, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain

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Product Information

86023-1-PBS targets CD74 as part of a matched antibody pair:

MP02221-1: 86023-2-PBS capture and 86023-1-PBS detection (validated in Cytometric bead array)

MP02221-2: 86023-1-PBS capture and 86023-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD74 fusion protein Eg1945
• Full Name: CD74 molecule, major histocompatibility complex, class II invariant chain
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: NM_001025159.3
• Gene Symbol: CD74
• Gene ID (NCBI): 972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04233-2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.