CD79a Polyclonal antibody

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  WB analysis using 31887-1-AP

  Mouse spleen tissue was subjected to SDS PAGE followed by western blot with 31887-1-AP (CD79A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 31887-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 31887-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 31887-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 31887-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 31887-1-AP (CD79A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat spleen using 31887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat spleen tissue using CD79a antibody (31887-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD79a Polyclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 31887-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

B-cell antigen receptor complex-associated protein alpha chain, Mb-1

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Tested Applications

• Positive WB detected in: mouse spleen tissue
• Positive IHC detected in: mouse spleen tissue, rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31887-1-AP targets CD79a in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD79a fusion protein Eg1388
• Full Name: CD79A antigen (immunoglobulin-associated alpha)
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_007655.3
• Gene Symbol: Cd79a
• Gene ID (NCBI): 12518
• RRID: AB_3670132
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P11911
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cell and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.