|Positive FC detected in||human PBMCs|
|This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.|
|Sample-dependent, check data in validation data gallery|
PE-65146 targets CD8 in FC applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG1, kappa|
|Full Name||CD8a molecule|
|Calculated molecular weight||235 aa, 26 kDa|
|GenBank accession number||BC025715|
|Gene ID (NCBI)||925|
|Conjugate||PE Fluorescent Dye|
|Excitation/Emission maxima wavelengths||496 nm, 565 nm / 578 nm|
|Purification Method||Affinity purification|
|Storage Buffer||PBS with 0.09% sodium azide and 0.5% BSA.|
|Storage Conditions||Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.|
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).