Rat CD80 Recombinant monoclonal antibody, PBS Only

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  WB analysis of NR8383 using 86532-1-RR (same clone as 86532-1-PBS)

  NR8383 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (CD80 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86532-1-PBS in a different storage buffer formulation.

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  WB analysis using 86532-1-RR (same clone as 86532-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (B7-1 / CD80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86532-1-PBS in a different storage buffer formulation.

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  WB analysis using 86532-1-RR (same clone as 86532-1-PBS)

  C2C12 cells were subjected to SDS PAGE followed by western blot with 86532-1-RR (B7-1 / CD80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86532-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 86532-1-RR (same clone as 86532-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using CD80 antibody (86532-1-RR, Clone: 250725H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86532-1-PBS in a different storage buffer formulation.

CD80 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86532-1-PBS

Clone No.250725H4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

B7 1, B7.1, B7-1, Cd80 molecule

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Product Information

86532-1-PBS targets CD80 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3841

  Product name: Recombinant Rat CD80 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 39-248 aa of

  Sequence: IVGQVSKSVREKALLSCDYKFCSEEQSIHRIYWQKHDKMVLSVISGVPEVWPEYKNRTVYDIANNYSFSLLGLILSDRGTYTCVVQRYEGGSYVVKHLTTVELSVRADFPTPNITESGNPSADIKRITCFASGGFPKPRLSWLENGRELNGINTTISQDPESELYTISSQLDFNTTYDHFIDCFIEYGDAHVSQNFTWEKPPEDPPDEKQ

• Full Name: Cd80 molecule
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 60-70 kDa
• Gene Symbol: CD80
• Gene ID (NCBI): 25408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q62680
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD80 (also known as B7-1) is a type I membrane protein that is a member of the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, monocytes, and macrophages. CD80 is the receptor for the proteins CD28 and CTLA-4 found on the surface of T-cells. It is involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. CD80 also acts as a cellular attachment receptor for adenovirus subgroup B. (PMID: 7545666; 12015893; 16920215)