FC experiment of Human Monocytes-derived dendritic cells using FITC-65171
1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 4 ul FITC Anti-Human CD83 (FITC-65171, Clone:HB15e) (green) or FITC mouse IgG1 isotype control (black). Cells were not fixed.
1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 4 ul FITC Anti-Human CD83 (FITC-65171, Clone:HB15e) (green) or FITC mouse IgG1 isotype control (black). Cells were not fixed.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.
Product Information
FITC-65171 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
FC experiment of Human Monocytes-derived dendritic cells using FITC-65171
1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 4 ul FITC Anti-Human CD83 (FITC-65171, Clone:HB15e) (green) or FITC mouse IgG1 isotype control (black). Cells were not fixed.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD83 Monoclonal antibody
Catalog Number
FITC-65171
Citations
-
Dilutions
Applications
FC
Reactivity
human, non-human primates
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
