Name: FITC Anti-Human CD83 (HB15e)
Brand: Proteintech
SKU: FITC-65171

Filter: AllFC

FC experiment of Human Monocytes-derived dendritic cells using FITC-65171
1X10^6 Human monocytes-derived dendritic cells (induced with GM-CSF+IL-4+TNF-α) were surface stained with 4 ul FITC Anti-Human CD83 (FITC-65171, Clone:HB15e) (green) or FITC mouse IgG1 isotype control (black). Cells were not fixed.

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Positive FC detected in

Human monocytes-derived dendritic cells

Application	Dilution
Flow Cytometry (FC)	FC : 10 ul per 10^6 cells in 100 μl suspension
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 4 µl per 10^6 cells in a 100 µl suspension or 4 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

FITC-65171 targets CD83 in FC applications and shows reactivity with human, non-human primates samples.

Tested Reactivity	human, non-human primates
Host / Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Immunogen	Human CD83-transfected Cos cells Predict reactive species
Full Name	CD83 molecule
Calculated Molecular Weight	205 aa, 23 kDa
GenBank Accession Number	BC030830
Gene Symbol	CD83
Gene ID (NCBI)	9308
ENSEMBL Gene ID	ENSG00000112149
RRID	AB_2920503
Conjugate	FITC Fluorescent Dye
Excitation/Emission Maxima Wavelengths	494 nm / 520 nm
Form	Liquid
Purification Method	The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT ID	Q01151
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Product Specific Protocols
FC protocol for FITC CD83 antibody FITC-65171	Download protocol

Standard Protocols
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