CD84 Recombinant monoclonal antibody

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  WB analysis using 84245-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84245-2-RR (CD84 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of Raji using 84245-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD84 antibody (84245-2-RR, Clone: 241342F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84245-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84245-2-RR against Human CD84 were performed. The affinity constant is 0.409 nM.

CD84 Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84245-2-RR

Clone No.241342F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

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Tested Applications

• Positive WB detected in: Raji cells, Daudi cells, Ramos cells, U-937 cells
• Positive IF/ICC detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84245-2-RR targets CD84 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD84 molecule
• Calculated Molecular Weight: 345 aa, 39 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC020063
• Gene Symbol: CD84
• Gene ID (NCBI): 8832
• RRID: AB_3671796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UIB8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).