Anti-Human CD84 Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98108-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR, Clone: 241345A3) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD4 (OKT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98108-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD84 Rabbit Recombinant Antibody (98108-1-RR, Clone: 241345A3) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98108-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98108-1-RR against Human CD84 were performed. The affinity constant is 1.14 nM.

CD84 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98108-1-RR

Clone No.241345A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Hly9-beta, Hly9 beta, hCD84, Cell surface antigen MAX.3, CD84 molecule

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98108-1-RR targets CD84 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD84 molecule
• Calculated Molecular Weight: 345 aa, 39 kDa
• GenBank Accession Number: BC020063
• Gene Symbol: CD84
• Gene ID (NCBI): 8832
• RRID: AB_3672255
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UIB8
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).