CD84 Recombinant antibody

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  WB analysis using 83828-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83828-4-RR (CD84/SLAMF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83828-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83828-4-RR against Mouse CD84/SLAMF5 were performed. The affinity constant is 1.77 nM.

CD84 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83828-4-RR

Clone No.240903D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CD84/SLAMF5, SLAMF5, CD84 antigen, 240903D6

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Tested Applications

• Positive WB detected in: Raji cells, THP-1 cells, Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83828-4-RR targets CD84 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD84 antigen
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 60 kDa
• Gene Symbol: Cd84
• Gene ID (NCBI): 12523
• RRID: AB_3671412
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q18PI6-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).