Anti-Mouse CD86 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98025-1-RR

  1x10^6 unstimulated or LPS stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 Rabbit Recombinant Antibody (98025-1-RR, Clone:230476B8) and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98025-1-RR

  1x10^6 unstimulated (black) or LPS stimulated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD86 Rabbit Recombinant Antibody (98025-1-RR, Clone:230476B8) and PE-Conjugated Goat Anti-Rabbit IgG (H+L) (red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98025-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98025-1-RR against Mouse CD86 were performed. The affinity constant is 25.4 nM.

CD86 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98025-1-RR

Clone No.230476B8

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

B7-2, B70, B7.2, B7 2, B7

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Tested Applications

• Positive FC detected in: LPS treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 9 publications below

Product Information

98025-1-RR targets CD86 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: CD86 antigen
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_019388
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• RRID: AB_3672169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P42082-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	FC	Acta Biochim Biophys Sin (Shanghai); Tissue extracellular vesicles suppress neonatal cardiac regeneration: a Pak2-Erk1/2-mediated macrophage paracrine signaling; Authors - Yongwei Li
mouse	FC	Mater Today Bio; 4D-printed Fe3O4-PLLA scaffolds modulate osteoimmune microenvironment for oral bone repair.; Authors - Wenlu Song
mouse	FC	Mater Today Bio; Biomimetic tumor cell membrane-camouflaged nanomicelles for synergistic chemo-immunotherapy of Triple-negative breast cancer.; Authors - Xiaoman Xu
mouse	FC	J Ethnopharmacol; Sijunzi decoction alleviates cancer cachexia myatrophy by suppressing M2 polarization of tumor-associated macrophages via the MCT1/lactate/H3K18 lactylation pathway.; Authors - Dengyun Nie
mouse	FC	J Nanobiotechnology; Extracellular vesicles derived from Kaempferia Galanga L. show promise for targeted oral therapy in the treatment of ulcerative colitis.; Authors - Lizhen Lin
mouse	FC	Int J Nanomedicine; Glioma Cell Membrane-Coated CaCO(3) Nanoparticles for Localized Postoperative Chemo-Calcium Overload Therapy to Prevent Glioma Recurrence.; Authors - Shiqiang Hou