CD89 Recombinant monoclonal antibody

• 

  WB analysis using 84384-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84384-5-RR (CD89 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84384-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84384-5-RR against Human CD89 were performed. The affinity constant is 13.5 nM.

CD89 Recombinant Antibody for WB, ELISA

Cat No. 84384-5-RR

Clone No.241426H1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IgA Fc receptor, FCAR, 241426H1

---

Tested Applications

• Positive WB detected in: A549 cells, HL-60 cells, K-562 cells, U-937 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84384-5-RR targets CD89 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: Fc fragment of IgA, receptor for
• Calculated Molecular Weight: 287 aa, 32 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC027953
• Gene Symbol: CD89
• Gene ID (NCBI): 2204
• RRID: AB_3671920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P24071
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FcαRI (CD89) is a member of the Fc receptor immunoglobulin superfamily, constitutively expressed on cells of the myeloid lineage like neutrophils, eosinophils, monocytes macrophages, and platelets (PMID: 28103138). CD89 expression and activation are increased in human blood phagocytes following bacterial infection, together with increased expression and association of FcRγ with CD89 (PMID: 34756952). CD89 binds both subclasses of IgA (IgA1 and IgA2) with moderate affinity, but also binds other molecules such as TfR1, C-reactive protein and bacterial ligands. CD89 represents an effective target molecule for immunotherapy mediated by bispecific antibodies (PMID: 37081893).