Anti-Mouse CD8a (53-6.7), PBS Only

• 

  FC experiment of C57BL/6 mouse splenocytes using 65069-1-Ig (same clone as 65069-1-PBS)

  1X10^6 C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (17A2) and 0.5 ug Anti-Mouse CD8a (65069-1-Ig, Clone:53-6.7) or 0.5 ug Rat IgG2a Isotype Control (2A3) and FITC anti-rat IgG2a Antibody at dilution 1:100. Cells were not fixed. This data was developed using the same antibody clone with 65069-1-PBS in a different storage buffer formulation.

CD8a Monoclonal Antibody for FC

Cat No. 65069-1-PBS

Clone No.53-6.7

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Synonyms

CD8, CD8 antigen, alpha chain, CD8α, Ly 2, Ly 35

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65069-1-Ig

Product Information

65069-1-PBS targets CD8a in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Spleen cells or thymocyte membranes

• Full Name: CD8 antigen, alpha chain
• GenBank Accession Number: BC030679
• Gene Symbol: Cd8a
• Gene ID (NCBI): 12525
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P01731
• Storage Buffer: PBS Only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer, while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).