Anti-Mouse CD8a (5H10-1), PBS Only

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• Anti-Mouse CD8a (5H10-1), PBS Only (65205-1-PBS)

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  IF Staining of mouse spleen using 65205-1-Ig (same clone as 65205-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD8a antibody (65205-1-Ig, Clone: 5H10-1 ) at dilution of 1:400 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65205-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse spleen using 65205-1-Ig (same clone as 65205-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD8a antibody (65205-1-Ig, Clone: 5H10-1 ) at dilution of 1:400 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 65205-1-PBS in a different storage buffer formulation.

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  FC experiment of C57BL/c mouse splenocytes using 65205-1-Ig (same clone as 65205-1-PBS)

  1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3ε and 0.25 ug Anti-Mouse CD8a (65205-1-Ig, Clone:5H10-1) and FITC anti-rat IgG2b Antibody at dilution 1:100, or rat IgG2b Isotype Control and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were not fixed. This data was developed using the same antibody clone with 65205-1-PBS in a different storage buffer formulation.

CD8a Monoclonal Antibody for IF-P, FC

Cat No. 65205-1-PBS

Clone No.5H10-1

Host / Isotype

Rat / IgG2b, lambda

Reactivity

mouse

Applications

IF-P, FC

Synonyms

CD8, 5H10-1, CD8 antigen, alpha chain, CD8α, Ly 2

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65205-1-Ig

Product Information

65205-1-PBS targets CD8a in IF-P, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Concanavalin A-stimulated BALB/c splenic T cells

• Full Name: CD8 antigen, alpha chain
• GenBank Accession Number: BC030679
• Gene Symbol: Cd8a
• Gene ID (NCBI): 12525
• RRID: AB_3084904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P01731
• Storage Buffer: PBS Only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).