Tested Applications
| Positive FC detected in | C57BL/6 mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-65205 targets CD8a in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2b, lambda |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Concanavalin A-stimulated BALB/c splenic T cells Predict reactive species |
| Full Name | CD8 antigen, alpha chain |
| GenBank Accession Number | BC030679 |
| Gene Symbol | Cd8a |
| Gene ID (NCBI) | 12525 |
| RRID | AB_3673993 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P01731 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD8a antibody PE-65205 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PE-65205 is an Anti-Mouse CD8a (5H10-1) monoclonal antibody. It has 2 citations. These appear in Advanced Science (IF 14.1) and International Journal of Biological Macromolecules (IF 8.7). The research fields covered are antitumor immunotherapy using multifunctional nano immunostimulants to overcome immunosuppressive microenvironments, and oral adjuvant strategies for enhancing intestinal mucosal and systemic immunity. Both studies applied the antibody in flow cytometry with mouse species.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Multifunctional Nano Immunostimulant: Overcoming Immunosuppressive Microenvironment for Antitumor Immunotherapy. | ||
Int J Biol Macromol The impact of novel oral adjuvant dendritic fibrous nano-silica coated with M cell homing peptide-modified chitosan on enhancing both the intestinal mucosal and systemic immunity. |
