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CD9 Monoclonal antibody
CD9 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human
Applications
WB, PLA, IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
4H7B9
Cat no : 60232-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, HeLa cells, A549 cells, L02 cells, HepG2 cells |
| Positive IHC detected in | human ovary tumor tissue, human colon cancer tissue, human tonsillitis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human breast cancer tissue, human ovary tumor tissue |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 58 publications below |
| IF | See 4 publications below |
| FC | See 1 publications below |
Product Information
60232-1-Ig targets CD9 in WB, PLA, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD9 fusion protein Ag14529 |
| Full Name | CD9 molecule |
| Calculated molecular weight | 228 aa, 25 kDa |
| Observed molecular weight | 23-27 kDa |
| GenBank accession number | BC011988 |
| Gene symbol | CD9 |
| Gene ID (NCBI) | 928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The cell-surface molecule CD9, a member of the transmembrane-4 superfamily, interacts with the integrin family and other membrane proteins, and is postulated to participate in cell migration and adhesion. Expression of CD9 enhances membrane fusion between muscle cells and promotes viral infection in some cells (PMID:10459022). It is often used as a mesenchymal stem cell marker (PMID:18005405). CD9 is also known as the p24 antigen besides MIC3, TSPAN29 because it is a protein of molecular weight 24 kD. The CD9 antigen appears to be a 227-amino acid molecule with 4 hydrophobic domains and 1 N-glycosylation site.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD9 antibody 60232-1-Ig | Download protocol |
| IHC protocol for CD9 antibody 60232-1-Ig | Download protocol |
| IF protocol for CD9 antibody 60232-1-Ig | Download protocol |
| FC protocol for CD9 antibody 60232-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Ther Single-cell dissection of cellular and molecular features underlying mesenchymal stem cell therapy in ischemic acute kidney injury | ||
Small Methods Highly Sensitive Exosome Detection for Early Diagnosis of Pancreatic Cancer Using Immunoassay Based on Hierarchical Surface-Enhanced Raman Scattering Substrate. | ||
Biomaterials Urinary exosomes-based Engineered Nanovectors for Homologously Targeted Chemo-Chemodynamic Prostate Cancer Therapy via abrogating IGFR/AKT/NF-kB/IkB signaling. | ||
Cell Death Dis Ficolin B secreted by alveolar macrophage exosomes exacerbates bleomycin-induced lung injury via ferroptosis through the cGAS-STING signaling pathway | ||
EMBO Mol Med SARS-CoV-2 spike protein enhances MAP4K3/GLK-induced ACE2 stability in COVID-19. | ||
Diabetes Exosomes From Adipose-Derived Stem Cells Attenuate Adipose Inflammation and Obesity Through Polarizing M2 Macrophages and Beiging in White Adipose Tissues. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Guorong (Verified Customer) (03-22-2022) | Very weak bands with strong background were detected at 25 kDa
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