Anti-Mouse CD90.2 (30-H12) Rat Recombinant Antibody, PBS Only

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• Anti-Mouse CD90.2 (30-H12) Rat Recombinant Antibody, PBS Only (65656-1-PBS)

CD90.2 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65656-1-PBS

Clone No.30-H12

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

CD90, Thy1, 30-H12, CD90/Thy1, Thy 1

• 

  FC experiment of mouse thymocytes using 65656-1-TR (same clone as 65656-1-PBS)

  1x10^6 C57BL/6 mouse thymocytes were surface stained with 0.25 ug Anti-Mouse CD90.2 (30-H12) Rat RecAb (65656-1-TR, Clone: 30-H12) (red) or 0.25 ug Isotype Control (blue), and Fluorescein (FITC)-conjugated Goat Anti-Mouse IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 65656-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

65656-1-PBS targets CD90.2 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: thymus cell antigen 1, theta
• GenBank Accession Number: BC054436
• Gene Symbol: CD90
• Gene ID (NCBI): 21838
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P01831
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).