FITC Plus Anti-Mouse CD90.2 (30-H12)

• 

  FC experiment of mouse splenocytes using FITC-65088

  1X10^6 mouse splenocytes were surface co-stained with Anti-Mouse CD3ε and 0.25 ug FITC Anti-Mouse CD90.2 (FITC-65088, Clone:30-H12) or 0.25 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of mouse thymocytes using FITC-65088

  1X10^6 mouse thymocytes were surface stained with 0.5 ug FITC Anti-Mouse CD90.2 (FITC-65088, Clone:30-H12) (red) or 0.5 ug Control Antibody. Cells were not fixed.

CD90.2 Monoclonal Antibody for FC

Cat No. FITC-65088

Clone No.30-H12

Publications(4)

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse and More (1)

Applications

FC

Synonyms

CD90, T25, Thy 1, Thy 1.2, Thy1, Thy1.1, Thy1.2, thymus cell antigen 1, theta

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Tested Applications

• Positive FC detected in: mouse splenocytes, mouse thymocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

FITC-65088 targets CD90.2 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Mouse thymus or spleen

• Full Name: thymus cell antigen 1, theta
• GenBank Accession Number: BC054436
• Gene Symbol: CD90
• Gene ID (NCBI): 21838
• RRID: AB_2883770
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P01831
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	FC	Stem Cell Res Ther; Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosis.; Authors - Xiaoyu Yang
mouse	FC	Arch Dermatol Res; METTL3-modified exosomes from adipose-derived stem cells enhance the proliferation and migration of dermal fibroblasts by mediating m6A modification of CCNB1 mRNA; Authors - Xiaolong Zhou
	FC	Exp Ther Med; Adipose-derived mesenchymal stem cell exosomes inhibit transforming growth factor-β1-induced collagen synthesis in oral mucosal fibroblasts.; Authors - Junjie Liu
		Commun Biol; FOXG1 promotes osteogenesis of bone marrow-derived mesenchymal stem cells by activating autophagy through regulating USP14; Authors - Yiqi Zhang