Anti-Mouse CD90.2 (30-H12)

CD90.2 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse and More (1)

Applications

FC

Conjugate

Unconjugated

Publications

1

CloneNo.

30-H12

Cat no : 65088-1-Ig

Synonyms

CD90, Thy1, Thy 1, 30-H12

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Validation Data Gallery

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  FC experiment of C57BL/6 mouse thymocytes using 65088-1-Ig

  1X10^6 C57BL/6 mouse thymocytes were surface stained with 1 ug Anti-Mouse CD90.2 (65088-1-Ig, Clone: 30-H12) (red) or 1 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2) (blue), and FITC anti-Rat IgG2b Antibody at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse thymocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

65088-1-Ig targets CD90.2 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: rat
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: thymus cell antigen 1, theta
• GenBank Accession Number: BC054436
• Gene Symbol: CD90
• Gene ID (NCBI): 21838
• RRID: AB_2918391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).

Publications

Species	Application	Title
rat	FC	Regen Ther; METTL3 promotes SMSCs chondrogenic differentiation by targeting the MMP3, MMP13, and GATA3; Authors - Bin Hu