CoraLite® Plus 647 Anti-Mouse CD90.2 (30-H12)

CD90.2 Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

30-H12

Cat no : CL647-65088

Synonyms

CD90, CD90.2, T25, Thy 1, Thy 1.2, Thy1, Thy1.1, Thy1.2, thymus cell antigen 1, theta

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL647-65088

  Immunofluorescent analysis of BALB/c mouse splenocytes using CoraLite® Plus 647 CD90.2 antibody (CL647-65088, Clone: 30-H12 ) at dilution of 1:100.

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  FC experiment of mouse thymocytes using CL647-65088

  1X10^6 BALB/c mouse thymocytes were surface stained with 0.5 ug CoraLite® Plus 647 Anti-Mouse CD90.2 (CL647-65088, Clone:30-H12) (red), or 0.5 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive IF detected in: BALB/c mouse splenocytes
• Positive FC detected in: BALB/c mouse thymocytes

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65088 targets CD90.2 in IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse thymus or spleen
• Full Name: thymus cell antigen 1, theta
• GenBank Accession Number: BC054436
• Gene Symbol: Thy1
• Gene ID (NCBI): 21838
• RRID: AB_2883674
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).