CoraLite® Plus 647 Anti-Mouse CD90.2 (30-H12)
CD90.2 Monoclonal Antibody for IF, FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
Mouse
Applications
IF, FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
30-H12
Cat no : CL647-65088
Synonyms
Validation Data Gallery
Tested Applications
Positive IF detected in | BALB/c mouse splenocytes |
Positive FC detected in | BALB/c mouse thymocytes |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL647-65088 targets CD90.2 in IF, FC applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Host / Isotype | Rat / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Mouse thymus or spleen |
Full Name | thymus cell antigen 1, theta |
GenBank Accession Number | BC054436 |
Gene Symbol | CD90.2 |
Gene ID (NCBI) | 21838 |
RRID | AB_2883674 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD90 (Thy-1) is a 25 kDa, GPI-linked membrane glycoprotein that belongs to immunoglobulin superfamily (PMID: 6177036; 6153212). Originally described as a brain thymus cross-reactive antigen, it is found in large quantities on mouse and rat thymocytes and central nervous system cells (PMID: 83175). CD90 has been postulated to be involved in cellular recognition, adherence, and T cell activation (PMID: 7683034). Mouse CD90 (Thy-1) is defined as a differentiation alloantigen, represented as two serologically distinguishable allelic forms, designated CD90.1 (Thy-1.1) and CD90.2 (Thy-1.2) (PMID: 83175). CD90.2 is expressed by thymocytes, T cells, neurons and hematopoietic stem cells in most strains of mice (C3H, BALB/c, C57BL/6, DBA, and others), whereas CD90.1 is expressed in some mouse strains like AKR (PMID: 5919593).
Protocols
Product Specific Protocols | |
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IF protocol for CL Plus 647 CD90.2 antibody CL647-65088 | Download protocol |
FC protocol for CL Plus 647 CD90.2 antibody CL647-65088 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |