Anti-Mouse CD94 (18D3) Rat Recombinant Antibody

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  FC experiment of mouse splenocytes using 65668-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD94 (18D3) Rat IgG2a RecAb (65668-1-TR, Clone:18D3) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1), and FITC anti-Rat IgG2a antibody. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD49b (HMA2) (CL647-65245, Clone: HMA2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65668-1-TR

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD94 (18D3) Rat IgG2a RecAb (65668-1-TR, Clone:18D3) (red) or Rat IgG2a Isotype Control Recombinant Antibody (98427-1-TR, Clone: 250862A1) (blue), and FITC anti-Rat IgG2a antibody. Cells were not fixed.

CD94 Uni-rAb^® Recombinant Antibody for FC

Cat No. 65668-1-TR

Clone No.18D3

Host / Isotype

Rat / IgG2a

Reactivity

mouse

Applications

FC

Synonyms

CD, Klrd1, 18D3, Killer cell lectin-like receptor subfamily D member 1

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65668-1-TR targets CD94 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a
• Class: Recombinant
• Type: Antibody
• Immunogen:

  N/A

• Full Name: killer cell lectin-like receptor, subfamily D, member 1
• GenBank Accession Number: BC120854
• Gene Symbol: CD94
• Gene ID (NCBI): 16643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O54707
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD94, also known as KLRD1, is a membrane protein that is preferentially expressed on NK cells. CD94 can form disulfide-bonded heterodimers with NKG2 family members. The CD94/NKG2A heterodimer constitutes an inhibitory receptor that recognizes HLA-E.