CD96 Polyclonal antibody

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  WB analysis using 34031-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 34031-1-AP (CD96 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

CD96 Polyclonal Antibody for WB, ELISA

Cat No. 34031-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

CD antigens, CD markers, CD molecule, CD molecules

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse liver tissue, mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

34031-1-AP targets CD96 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg5283

  Product name: Recombinant mouse CD96 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 22-536 aa of NM_032465.2

  Sequence: VWEELFNVGDDVYALPGSDINLTCQTKEKNFLVQMQWSKVTDKNDMIALYHPQYGLYCGQEHACESQVAATETEKGVTNWTLYLRNISSALGGKYECIFTLYPEGIKTTVYNLIVEPYTQDEHNYTIEIETNRTLEIPCFQNTSSEIPPRFTFSWLVEKDGVEEVLFTHHHHVNNSTSFKGRIRLGGDYRLHLSPVQIQDDGRTFSCHLTVNPLKAWKMSTTVKVFAKPEILMTVENSTMDVLGERVFTCLLKNVFPKANITWFIDGRFLQGNEEGIYITNEEKNCSSGFWELKSVLTRMHSGPSQSNNMTAWCMALSPGPRNKMWNTSSQPITVSFDSVIAPTKHLPTVTGSTLGTQPFSDAGVSPTGYLATPSVTIVDENGLTPDATPQTSNSSMTTKDGNYLEASSGTDAKNSSRAAASSKSGSWPFPFTSPPEWHSLPGTSTGPQEPDSPVSWIPSEVHTSAPLDASLAPHDTIISTTTEFPNVLTTANGTTKIDHGPITSIIVNQPSDGM

• Full Name: CD96 antigen
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: NM_032465.2
• Gene Symbol: Cd96
• Gene ID (NCBI): 84544
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q3U0X8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD96 (also known as TACTILE) is a type I trans-membrane glycoprotein of the immunoglobulin superfamily (IgSF). Expression is largely restricted to activated T cells, NK cells and a subset of NKT cells. On these cells CD96 functions as an adhesion and immunomodulatory receptor that binds the nectin-family ligand CD155 (PVR) with high affinity. (PMID: 30528596)