CD97 Polyclonal antibody
CD97 Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
WB, IHC, FC, ELISA
Cat no : 13071-1-AP
Validation Data Gallery
|Positive WB detected in||HepG2 cells, K-562 cells, U-937 cells, Jurkat cells|
|Positive IHC detected in||human tonsillitis tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||K-562 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
13071-1-AP targets CD97 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD97 fusion protein Ag3745|
|Full Name||CD97 molecule|
|Calculated molecular weight||722 aa, 79 kDa|
|Observed molecular weight||75-85 kDa|
|GenBank accession number||BC026690|
|Gene ID (NCBI)||976|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Fiona (Verified Customer) (01-09-2020)
Used sample to screen cell lines for expression of the CD97 protein. Didn't observe band where expected for U-937 cells (positive control) however assay requires further optimisation. Otherwise blot was clean. Specific clean staining observed when used for immunofluorescence.