CD97 Polyclonal antibody

CD97 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG







Cat no : 13071-1-AP


CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, TM7LN1

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, U-937 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13071-1-AP targets CD97 in WB, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD97 fusion protein Ag3745
Full Name CD97 molecule
Calculated Molecular Weight 722 aa, 79 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberBC026690
Gene Symbol CD97
Gene ID (NCBI) 976
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD97 is a member of the seven-trans-membrane subfamily of the class B G protein-coupled receptor (GPCR) group of the epidermal growth factor (EGF) and is present on the surface of lymphocytes, monocytes, macrophages, dendritic cells, granulocytes, and smooth muscle, being a dimeric glycoprotein with a 75-90 kDa intracellular domain and a 28 kDa extracellular domain (PMID: 23969601). It plays a vital role in cell adhesion, migration, and cell connection regulation (PMID: 36464675). CD97 is expressed on T-cells, but rarely on B cells. CD97 might play key roles in cellular adhesion, through connections with other proteins of the cell surface and of the extracellular matrix (PMID: 32781778).


Product Specific Protocols
WB protocol for CD97 antibody 13071-1-APDownload protocol
IHC protocol for CD97 antibody 13071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Fiona (Verified Customer) (01-09-2020)

Used sample to screen cell lines for expression of the CD97 protein. Didn't observe band where expected for U-937 cells (positive control) however assay requires further optimisation. Otherwise blot was clean. Specific clean staining observed when used for immunofluorescence.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Multiple cell lines