Mouse CD98/SLC3A2 Recombinant monoclonal antibody, PBS Only

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  WB analysis of mouse kidney using 84040-7-RR (same clone as 84040-7-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 84040-7-RR (same clone as 84040-7-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84040-7-RR (CD98 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84040-7-RR (same clone as 84040-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 84040-7-RR (same clone as 84040-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84040-7-RR (same clone as 84040-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84040-7-RR (same clone as 84040-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84040-7-RR (CD98 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84040-7-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84040-7-RR

  Biolayer interferometry (BLl) kinetic assays of 84040-7-RR against Mouse CD98/SLC3A2 were performed. The affinity constant is 1.36 nM.

CD98/SLC3A2 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84040-7-PBS

Clone No.241247F12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

4F2 cell-surface antigen heavy chain, 4F2hc, Amino acid transporter heavy chain SLC3A2, CD98, Mdu1

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Product Information

84040-7-PBS targets CD98/SLC3A2 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1374

  Product name: Recombinant Mouse CD98 protein (His Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: N-6*His

  Domain: 100-526 aa of NM_008577.4

  Sequence: APRCRELPVQRWWHKGALYRIGDLQAFVGRDAGGIAGLKSHLEYLSTLKVKGLVLGPIHKNQKDEINETDLKQINPTLGSQEDFKDLLQSAKKKSIHIILDLTPNYQGQNAWFLPAQADIVATKMKEALSSWLQDGVDGFQFRDVGKLMNAPLYLAEWQNITKNLSEDRLLIAGTESSDLQQIVNILESTSDLLLTSSYLSNSTFTGERTESLVTRFLNATGSQWCSWSVSQAGLLADFIPDHLLRLYQLLLFTLPGTPVFSYGDELGLQGALPGQPAKAPLMPWNESSIFHIPRPVSLNMTVKGQNEDPGSLLTQFRRLSDLRGKERSLLHGDFHALSSSPDLFSYIRHWDQNERYLVVLNFRDSGRSARLGASNLPAGISLPASAKLLLSTDSARQSREEDTSLKLENLSLNPYEGLLLQFPFVA

• Full Name: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: NM_008577.4
• Gene Symbol: CD98/SLC3A2
• Gene ID (NCBI): 17254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P10852-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).