Tested Applications
Positive WB detected in | Jurkat cells, MCF-7 cells, T-47D cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
32010-1-AP targets CD98/SLC3A2 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CD98/SLC3A2 fusion protein Eg1948 Predict reactive species |
Full Name | solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 |
Calculated Molecular Weight | 68kDa |
Observed Molecular Weight | 85-94 kDa, 120-130 kDa |
GenBank Accession Number | NM_002394.5 |
Gene Symbol | CD98 |
Gene ID (NCBI) | 6520 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | P08195-1 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).
Protocols
Product Specific Protocols | |
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WB protocol for CD98/SLC3A2 antibody 32010-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |