Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, HepG2 cells, HT-29 cells, COLO 320 cells, Caco-2 cells, Jurkat cells |
| Positive IHC detected in | human renal cell carcinoma tissue, human colon cancer tissue, human cervical cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66883-1-Ig targets CD98/SLC3A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7301 Product name: Recombinant human SLC3A2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 269-630 aa of BC001061 Sequence: KDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA Predict reactive species |
| Full Name | solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 |
| Calculated Molecular Weight | 68 kDa |
| Observed Molecular Weight | 85-94 kDa, 120-130 kDa |
| GenBank Accession Number | BC001061 |
| Gene Symbol | CD98 |
| Gene ID (NCBI) | 6520 |
| RRID | AB_2882215 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P08195 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CD98/SLC3A2 antibody 66883-1-Ig | Download protocol |
| IHC protocol for CD98/SLC3A2 antibody 66883-1-Ig | Download protocol |
| WB protocol for CD98/SLC3A2 antibody 66883-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CD98/SLC3A2 Monoclonal antibody (2B10F5), catalog number 66883-1-Ig, has 11 citations published in journals including Nature Microbiology, Nature Communications, Science Advances, PNAS, Journal of Extracellular Vesicles, Apoptosis, Phytomedicine, Cancer Cell International, Ecotoxicology and Environmental Safety, Respiratory Research, and Oncology Letters. Research fields span exosome biology, ferroptosis, cancer metabolism, virology, miRNA regulation, and inflammatory mechanisms.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Homosalate boosts the release of tumour-derived extracellular vesicles with protection against anchorage-loss property. | ||
Nat Microbiol Mapping in-cell protein contact sites reveals hijacking of paraspeckles during influenza A virus infection. | ||
Nat Commun Specificities of exosome versus small ectosome secretion revealed by live intracellular tracking of CD63 and CD9. | ||
Sci Adv Screening the human miRNA interactome reveals coordinated up-regulation in melanoma, adding bidirectional regulation to miRNA networks | ||
Phytomedicine Identification of the target protein and molecular mechanism of honokiol in anti-inflammatory action | ||
Proc Natl Acad Sci U S A Activation of polyamine catabolism promotes glutamine metabolism and creates a targetable vulnerability in lung cancer |
