CDAN1 Polyclonal antibody

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  WB analysis of HeLa using 17597-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17597-1-AP (CDAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17597-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17597-1-AP (CDAN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 17597-1-AP

  IP result of anti-CDAN1 (IP:17597-1-AP, 4ug; Detection:17597-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IF Staining of HeLa using 17597-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17597-1-AP (CDAN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CDAN1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 17597-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Codanin 1, codanin, CDAN 1, CDAI, CDA1

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Tested Applications

• Positive WB detected in: HeLa cells, K-562 cells
• Positive IP detected in: K-562 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

17597-1-AP targets CDAN1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11589

  Product name: Recombinant human CDAN1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 881-1228 aa of BC066640

  Sequence: IKATLVADLVRQAESLLQEQLVTQGEEGGDPAQLLEILCSQLCPHGAQALALGREFCQRKSPGAVRALLPEETPAAVLSSAENIAVGLATEKACAWLSANITALIRREVKAAVSRTLRAQGPEPAARGERRGCSRACEHHAPLPSHLISEIKDVLSLAVGPRDPDEGVSPEHLEQLLGQLGQTLRCRQFLCPPAEQHLAKCSVELASLLVADQIPILGPPAQYRLERGQARRLLHMLLSLWKEDFQGPVPLQLLLSPRNVGLLADTRPREWDLLLFLLRELVEKGLMGRMEIEACLGSLHQAQWPGDFAEELATLSNLFLAEPHLPEPQLRACELVQPNRGTVLAQS

• Full Name: congenital dyserythropoietic anemia, type I
• Calculated Molecular Weight: 1227 aa, 134 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC066640
• Gene Symbol: CDAN1
• Gene ID (NCBI): 146059
• RRID: AB_10694142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IWY9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDAN1 acts as a negative regulator of ASF1 in chromatin assembly. It appears to play a role in nuclear envelope integrity, possibly related to microtubule attachments. Mutations of CDAN1 cause congenital dyserythropoietic anemia type I, a disease resulting in morphological and functional abnormalities of erythropoiesis. There're some isoforms this gene.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Biochem J; A complex comprising C15ORF41 and Codanin-1: the products of two genes mutated in congenital dyserythropoietic anaemia type I (CDA-I).; Authors - Maithili Shroff