CDC23 Polyclonal antibody

CDC23 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10683-1-AP

Synonyms

ANAPC8, APC8, CDC23, Cyclosome subunit 8

---

Validation Data Gallery

• 

  WB analysis using 10683-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10683-1-AP (CDC23 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: MOLT-4 cells, HEK-293 cells, Jurkat cells, K-562 cells, Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10683-1-AP targets CDC23 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC23 fusion protein Ag1068
• Full Name: cell division cycle 23 homolog (S. cerevisiae)
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 69 kDa
• GenBank Accession Number: BC005258
• Gene Symbol: CDC23
• Gene ID (NCBI): 8697
• RRID: AB_2075008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC23(cell division cycle protein 23 homolog) is also named ANAPC8 and belongs to the APC8/CDC23 family. It is a subunit of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. It can be phosphorylated and the phosphorylation of Thr-562 occurs specifically during mitosis(PMID:19690332). It has 3 isoforms produced by alternative splicing with the molecular weight of 69 kDa, 17 kDa, and 56 kDa.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC; Authors - Qi Li