CDK8/19 Polyclonal antibody

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  WB analysis of Y79 using 13761-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDK8/19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13761-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDC2L6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13761-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13761-1-AP (CDK8/19 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 13761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13761-1-AP (CDK8/19 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 13761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13761-1-AP (CDK8/19 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CDK8/19 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13761-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CDC2L6, CDC2 related protein kinase 6, CDC2-related protein kinase 6, CDK11, CDK19

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Tested Applications

• Positive WB detected in: Y79 cells, mouse brain tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

13761-1-AP targets CDK8/19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4731

  Product name: Recombinant human CDC2L6 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-335 aa of BC037289

  Sequence: MDYDFKAKLAAERERVEDLFEYEGCKVGRGTYGHVYKARRKDGKDEKEYALKQIEGTGISMSACREIALLRELKHPNVIALQKVFLSHSDRKVWLLFDYAEHDLWHIIKFHRASKANKKPMQLPRSMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPFHHDQLDRIFSVMGFPADKDWEDIRKMPEYPTLQKDFRRTTYANSSLIKYMEKHKVKPDSKVFLLLQKLLTMDPTKRITSEQALQDPYF

• Full Name: cell division cycle 2-like 6 (CDK8-like)
• Calculated Molecular Weight: 502 aa, 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC037289
• Gene Symbol: CDK19
• Gene ID (NCBI): 23097
• RRID: AB_2276048
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BWU1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CDK8 homolog, CDK19 (Cdk11, CDC2L6) was discovered by Hidden Markov model (HMM) profiling of a eukaryotic protein kinase domain, and found to have approximately 91% sequence homology to Cdk8. The two proteins share a particularly high degree of sequence conservation in their kinase and cyclin binding domain (PMID: 30693281, 26985305). CDK8 and CDK19, collectively termed 'Mediator Kinase,' are incorporated, in a mutually exclusive manner, as part of a 4-protein complex called the Mediator kinase module. CDK8/19 inhibition suppressed the induction of NFκB-driven transcription by p21 (PMID: 28855340).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Cell Mol Life Sci; Hotair promotes the migration and proliferation in ovarian cancer by miR-222-3p/CDK19 axis.; Authors - Lili Fan