|Positive WB detected in||HT-29 cells, HeLa cells, C6 cells, Jurkat cells|
|Positive IHC detected in||human pancreas cancer tissue, human pancreas tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C6 cells, HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10155-1-AP targets CDC42 in WB, IHC, IF, CoIP applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CDC42 fusion protein Ag0207|
|Full Name||cell division cycle 42 (GTP binding protein, 25kDa)|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC002711|
|Gene ID (NCBI)||998|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Cdc42, the human homologue of the cell division cycle 42 of Saccharomyces cerevisiae, belongs to the Rho/Rac subfamily of Ras-like GTPases. It acts as molecular switches to regulate numerous cellular responses including the organization of the actin cytoskeleton, gene transcription, cell cycle progression, and membrane trafficking. The molecular weight is about 21 kd.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ambra (Verified Customer) (01-19-2021)
I used it 1:500 in 5% milk in TBS-tween to have a nice signal in Human Brain lysate.The signal is quite low used 1:1000.