Tested Applications
| Positive WB detected in | K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse thymus tissue |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15678-1-AP targets CDC45L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8150 Product name: Recombinant human CDC45L protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 217-566 aa of BC010022 Sequence: LWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS Predict reactive species |
| Full Name | CDC45 cell division cycle 45-like (S. cerevisiae) |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 60-66 kDa |
| GenBank Accession Number | BC010022 |
| Gene Symbol | CDC45L |
| Gene ID (NCBI) | 8318 |
| RRID | AB_10642704 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75419 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation. This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDC45L antibody 15678-1-AP | Download protocol |
| IHC protocol for CDC45L antibody 15678-1-AP | Download protocol |
| IP protocol for CDC45L antibody 15678-1-AP | Download protocol |
| WB protocol for CDC45L antibody 15678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDC45L Polyclonal antibody (Cat# 15678-1-AP) has 11 total citations published in journals including Signal Transduction and Targeted Therapy, Nature Communications, Nucleic Acids Research, Advanced Science, EMBO Journal, European Journal of Medicinal Chemistry, Oncotarget, Cell Cycle, and Annals of Translational Medicine. Research fields span DNA replication origin firing, CDK regulation, R-loop accumulation, and cancer biology (non-small cell lung cancer, breast cancer, hepatocellular carcinoma).
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther LSD1 is required for euchromatic origin firing and replication timing. | ||
Nat Commun Dynamic regulation of origin firing factors links CDK activity to dormant origin activation. | ||
Nucleic Acids Res The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phase.
| ||
Adv Sci (Weinh) Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage | ||
Eur J Med Chem Design and synthesis of novel 1,2,4-triazolobenzene sulfonamide derivatives as selective CDK1 inhibitors with potent in vivo anticancer efficacy.
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