CDC45L Polyclonal antibody

CDC45L Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15678-1-AP

Synonyms

CDC45, CDC45L, CDC45L2, PORC PI 1



Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse thymus tissue
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15678-1-AP targets CDC45L in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC45L fusion protein Ag8150
Full Name CDC45 cell division cycle 45-like (S. cerevisiae)
Calculated molecular weight 64 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC010022
Gene symbol CDC45L
Gene ID (NCBI) 8318
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation. This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L.

Protocols

Product Specific Protocols
WB protocol for CDC45L antibody 15678-1-APDownload protocol
IHC protocol for CDC45L antibody 15678-1-APDownload protocol
IF protocol for CDC45L antibody 15678-1-APDownload protocol
IP protocol for CDC45L antibody 15678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

LSD1 is required for euchromatic origin firing and replication timing.

Authors - Yue Wang
humanIHC

Ann Transl Med

Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.

Authors - Wenhui Xie
humanWB

Oncotarget

The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.

Authors - Lifei Fan
humanWB

Nucleic Acids Res

The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phase.

Authors - Kimberlie A Wittig
  • KD Validated