CDC45L Polyclonal antibody

• Featured Product
• KD/KO Validated

CDC45L Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15678-1-AP

Synonyms

CDC45, CDC45L, CDC45L2, PORC PI 1

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Validation Data Gallery

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  WB analysis of K-562 using 15678-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15678-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15678-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15678-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 15678-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 15678-1-AP (CDC45L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 15678-1-AP

  IP result of anti-CDC45L (IP:15678-1-AP, 3ug; Detection:15678-1-AP 1:1000) with K-562 cells lysate 3400ug.

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  IHC staining of mouse testis using 15678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15678-1-AP (CDC45L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15678-1-AP (CDC45L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15678-1-AP

  Immunofluorescent analysis of Hela cells, using CDC45L antibody 15678-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15678-1-AP

  1X10^6 HeLa cells were stained with 0.2ug CDC45L antibody (15678-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, HeLa cells, Jurkat cells, mouse thymus tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

15678-1-AP targets CDC45L in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CDC45L fusion protein Ag8150
• Full Name: CDC45 cell division cycle 45-like (S. cerevisiae)
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 60-66 kDa
• GenBank Accession Number: BC010022
• Gene Symbol: CDC45L
• Gene ID (NCBI): 8318
• RRID: AB_10642704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation. This is a rabbit polyclonal antibody raised against part of C-terminus of human CDC45L.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; The CRL4DTL E3 ligase induces degradation of the DNA replication initiation factor TICRR/TRESLIN specifically during S phase.; Authors - Kimberlie A Wittig ; KD Validated
human	WB	Oncotarget; The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.; Authors - Lifei Fan
human	IHC	Ann Transl Med; Nine hub genes related to the prognosis of HBV-positive hepatocellular carcinoma identified by protein interaction analysis.; Authors - Wenhui Xie
human	WB	Signal Transduct Target Ther; LSD1 is required for euchromatic origin firing and replication timing.; Authors - Yue Wang
human	WB	Cell Cycle; IGF2BP2 promotes glycolysis and hepatocellular carcinoma stemness by stabilizing CDC45 mRNA via m6A modification; Authors - Tao Wu ; KD Validated