CDC45L Recombinant monoclonal antibody

• 

  WB analysis using 87219-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87219-1-RR (CDC45L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CDC45L Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87219-1-RR

Clone No.252457D11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

CDC45, CDC45L2, PORC PI 1, PORC-PI-1, UNQ374/PRO710

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cell, Jurkat cell, Raji cell, K-562 cell, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87219-1-RR targets CDC45L in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8150

  Product name: Recombinant human CDC45L protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 217-566 aa of BC010022

  Sequence: LWWAIVGLTDQWVQDKITQMKYVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTAARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMRVQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLELAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSFVCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRMLHNHFDLSVIELKAEDRSKFLDALISLLS

• Full Name: CDC45 cell division cycle 45-like (S. cerevisiae)
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC010022
• Gene Symbol: CDC45L
• Gene ID (NCBI): 8318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75419
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDC45L(Cell division control protein 45 homolog), plays a role in the initiation of DNA replication, and genetically interacts with MCM family members and with ORC2 and physically form a complex including Mcm5p. It's the first gene missed in DiGeorge syndrome that suggest involve in cell proliferation.