Tested Applications
| Positive WB detected in | human brain tissue, HEK-293 cells, HepG2 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12974-1-AP targets CDC5L in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3578 Product name: Recombinant human CDC5L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 451-803 aa of BC001568 Sequence: NPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF Predict reactive species |
| Full Name | CDC5 cell division cycle 5-like (S. pombe) |
| Calculated Molecular Weight | 802 aa, 92 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC001568 |
| Gene Symbol | CDC5L |
| Gene ID (NCBI) | 988 |
| RRID | AB_2078510 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99459 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CDC5L antibody 12974-1-AP | Download protocol |
| IHC protocol for CDC5L antibody 12974-1-AP | Download protocol |
| IP protocol for CDC5L antibody 12974-1-AP | Download protocol |
| WB protocol for CDC5L antibody 12974-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDC5L Polyclonal antibody (Cat# 12974-1-AP) has 8 citations published in journals including Apoptosis, Int J Mol Med, EMBO Mol Med, Oncotarget, Acta Biochim Biophys Sin (Shanghai), J Biol Chem, Cancer Med, and Cell Oncol (Dordr). Research fields span acute myeloid leukemia, hepatocellular carcinoma, melanoma, ovarian cancer, NSCLC angiogenesis, cardiomyocyte proliferation, O-GlcNAcylation, and alternative splicing in fertility. Applications cited include WB, IHC, IF, CoIP, and RIP in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Apoptosis LncRNA AC098613.1 promotes acute myeloid leukemia cell differentiation through CDC5L/ADAP1/NRD1 axis. | ||
Int J Mol Med CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.
| ||
EMBO Mol Med CCDC174 deficiency impaired human fertility by affecting the alternative splicing of maternal mRNAs. | ||
Acta Biochim Biophys Sin (Shanghai) CDC5L facilitates cardiomyocyte proliferation and ameliorates myocardial ischemia-reperfusion injury via modulation of the FGF10-YAP axis. | ||
J Biol Chem O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity. |
Reviews
What customers say
The single review for this product is a 5-star rating from a Western Blot application. The user tested it at a 1:2000 dilution on Hek293 cell lysates and reported clear detection of CDC5L in both nuclear and cytoplasmic fractions, with a specific band observed between 100 and 130 kDa. Overall, the feedback highlights strong specificity and reliable performance for WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wael (Verified Customer) (01-19-2023) | CDC5L detected in both nuclear and cytoplasmic fractions of Hek293 lysates. specific band between 100 and 130kd.
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