CDC5L Polyclonal antibody

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  WB analysis of human brain using 12974-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12974-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12974-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12974-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12974-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12974-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12974-1-AP

  IP result of anti-CDC5L (IP:12974-1-AP, 4ug; Detection:12974-1-AP 1:500) with mouse brain tissue lysate 1600 ug.

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  IHC staining of mouse testis using 12974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 12974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12974-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12974-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (12974-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CDC5L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12974-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PCDC5RP, KIAA0432, Cell division cycle 5-like protein, CEF1, Cdc5-like protein

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Tested Applications

• Positive WB detected in: human brain tissue, HEK-293 cells, HepG2 cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12974-1-AP targets CDC5L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3578

  Product name: Recombinant human CDC5L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 451-803 aa of BC001568

  Sequence: NPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF

• Full Name: CDC5 cell division cycle 5-like (S. pombe)
• Calculated Molecular Weight: 802 aa, 92 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC001568
• Gene Symbol: CDC5L
• Gene ID (NCBI): 988
• RRID: AB_2078510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99459
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
	WB	J Biol Chem; O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity.; Authors - Li Liu
human	WB	Cancer Med; Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway.; Authors - Chunmei Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wael (Verified Customer) (01-19-2023)

CDC5L detected in both nuclear and cytoplasmic fractions of Hek293 lysates. specific band between 100 and 130kd.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Hek293

CDC5L Antibody Western Blot validation (1:2000 dilution) in Hek293 (Cat no:12974-1-AP)