HRPT2, CDC73 Polyclonal antibody

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• KD/KO Validated

HRPT2, CDC73 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12310-1-AP

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, Hyperparathyroidism 2 protein, Parafibromin

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Validation Data Gallery

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  WB analysis using 12310-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12310-1-AP (HRPT2, CDC73 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12310-1-AP

  WB result of HRPT2, CDC73 antibody (12310-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HEK-293 cells.

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  IP experiment of mouse testis using 12310-1-AP

  IP result of anti-HRPT2, CDC73 (IP:12310-1-AP, 4ug; Detection:12310-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human breast cancer using 12310-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12310-1-AP (HRPT2, CDC73 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12310-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12310-1-AP (HRPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 12310-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRPT2, CDC73 antibody (12310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IP: See 1 publications below

Product Information

12310-1-AP targets HRPT2, CDC73 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HRPT2, CDC73 fusion protein Ag2959
• Full Name: cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 531 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC014351
• Gene Symbol: HRPT2/CDC73
• Gene ID (NCBI): 79577
• RRID: AB_2078388
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).

Publications

Species	Application	Title
mouse	WB,IP	Cell; Inherited blood cancer predisposition through altered transcription elongation; Authors - Jiawei Zhao