• Featured Product
  • KD/KO Validated

HRPT2, CDC73 Polyclonal antibody

HRPT2, CDC73 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12310-1-AP

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, Hyperparathyroidism 2 protein, Parafibromin



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12310-1-AP targets HRPT2, CDC73 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HRPT2, CDC73 fusion protein Ag2959
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated Molecular Weight 531 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC014351
Gene Symbol CDC73
Gene ID (NCBI) 79577
RRIDAB_2078388
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).

Protocols

Product Specific Protocols
WB protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IHC protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IF protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IP protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Cell

Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao