CDCP1/CD318 Recombinant monoclonal antibody

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• CDCP1/CD318 Recombinant monoclonal antibody (87514-4-RR)

CDCP1/CD318 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87514-4-RR

Clone No.251667H4

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CD318, Cdcp1, CUB domain-containing protein 1, Membrane glycoprotein gp140, Transmembrane and associated with src kinases

• 

  WB analysis using 87514-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87514-4-RR (CDCP1/CD318 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 87514-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87514-4-RR (CDCP1/CD318 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse colon tissue, rat skin tissue, rat colon tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87514-4-RR targets CDCP1/CD318 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6199

  Product name: Recombinant mouse CD318/CDCP1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 30-666 aa of NM_133974.3

  Sequence: SEIALPQRSGVTVSIKLGNPALPVKICYIVMSRQHITELIIRPGERKSFTFSCSNPEKHFVLKIEKNIDCMSGPCPFGEVHLQPSTSELPILNRTFIWDVRAHKSIGLELQFATPRLRQIGPGESCADGVTHSISGHIDATEVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHRRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPGGFPEDELMTWQFVVPAHLRASVSFLNFNVSNCERKEERVEYYIPGSTTNPEVFRLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQRPQDESNKTYMVDLSRERTMSLTIEPRPVKHGRRFVPGCFVCLESRTCSTNVTLTAGSIHKISFLCDDLTRLWVNVEKTLSCLDHRYCYRQSFKLQVPDYILQLPVQLHDFSWKLLVPKDKLSLMLVPGQKLQQHTQERPCNTSFGYHVTSTTPGQDLYFGSFCSGGSIEKIQVKQNSSVTLRAYAPSFQQEVSKQGLIVSYTPYFKEEGIFTVTPDTKNKVYLRSPNWDRGLPALSSVSWNISVPSNQVACLTVLKERSGLACQSGRAFMIIQEQQSRAEEIFSLEEEVLPKPSFHHHSFWVNISNCSPMNGKQLDLLFWVTLTPRTVDL

• Full Name: CUB domain containing protein 1
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: NM_133974.3
• Gene Symbol: Cdcp1
• Gene ID (NCBI): 109332
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5U462
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD318 (also known as CDCP1, TRASK, SIMA135, or gp140) is a cell-surface glycoprotein with an apparent molecular mass of ∼140 kDa. It consists of three extracellular CUB domains, a transmembrane domain, and an intracellular domain. CD318 can be proteolytically cleaved between the two distal CUB domains by certain serine proteases, resulting in different ratios of the ∼140-kDa intact molecule and the ∼80-kDa cleaved product on various cells. Cleaved CD318 is phosphorylated and activated by Src kinase, then the activated CD318 forms a complex with activated β1 integrin and activates FAK/PI3K/Akt motility signaling to promote early tumor dissemination. Under normal conditions, CD318 is present on many epithelial cells, some hematopoietic cells (18), and mesenchymal stem cells. CD318 is also present on many tumor cells. Up-regulation of CD318 expression is associated with a poor prognosis for many cancer patients.