Cadherin-11 Polyclonal antibody

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  WB analysis of PC-3 using 12028-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12028-1-AP (Cadherin-11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Cadherin-11 Polyclonal Antibody for WB, ELISA

Cat No. 12028-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CDH11, CAD11, Cadherin 11, CDHOB, OB

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Tested Applications

• Positive WB detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12028-1-AP targets Cadherin-11 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2659

  Product name: Recombinant human CDH11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 29-380 aa of BC013609

  Sequence: GHLRPSFHGHHEKGKEGQVLQRSKRGWVWNQFFVIEEYTGPDPVLVGRLHSDIDSGDGNIKYILSGEGAGTIFVIDDKSGNIHATKTLDREERAQYTLMAQAVDRDTNRPLEPPSEFIVKVQDINDNPPEFLHETYHANVPERSNVGTSVIQVTASDADDPTYGNSAKLVYSILEGQPYFSVEAQTGIIRTALPNMDREAKEEYHVVIQAKDMGGHMGGLSGTTKVMITLTDVNDNPPKFPQSVYQMSVSEAAVPGEEVGRVKAKDPDIGENGLVTYNIVDGDGMESFEITTDYETQEGVIKLKKPVDFETKRAYSLKVEAANVHIDPKFISNGPFKDTVTVKIAVEDADEP

• Full Name: cadherin 11, type 2, OB-cadherin (osteoblast)
• Calculated Molecular Weight: 796 aa, 88 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC013609
• Gene Symbol: CDH11
• Gene ID (NCBI): 1009
• RRID: AB_3085386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55287
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-11 (CDH11), also known osteoblast cadherin (OB-cadherin) or OSF-4, was identified initially in a mouse osteoblastic cell line and human osteosarcoma (PMID: 8163513). Cadherin-11 is a single pass transmembrane protein consisting of an extracellular domain which contains five repeat sequences (EC1-EC5), a transmembrane segment and a cytoplasmic domain which links catenins to the actin and microtubule cytoskeleton (PMID: 8163513; 33442417). It has been shown that cadherin-11 plays important roles in the occurrence and development of a lot of diseases, arthritis, cancer, aortic calcification disease, dermatosis (PMID: 33442417).